Reference | MBTNJ9HN (86)

Details of animal trial design and management were previously described (74). A total of 1,600 1-day-old male Cobb 500 broiler chickens were allocated into 20 floor pens (80 birds per pen) and vaccinated against coccidiosis with a commercial vaccine. Birds in each group were distributed into five dietary treatments (four pens per treatment): (i) basal diet as a negative control (NC); (ii) basal diet with 55 ppm of BAC methylene disalicylate; (iii) basal diet with 100 ppm of encapsulated CIN; (iv) basal diet with100 ppm of encapsulated CIT; and (v) basal diet with a combination of 100 ppm each of encapsulated CIN and CIT (CINþCIT). Birds were fed the five diets during their three growth phases: day 0 to day 9 (starter phase), day 10 to day 19 (grower phase), and day 20 to day 28 (finisher phase). The basal diet contained primarily cereals and soybean meals and was formulated and pelleted according to Cobb 500 guidelines as previously described.

Fresh fecal samples were collected on days 6, 16, 23, and 27 from all 20 pens (three birds per pen ¼ 60 samples) in sterilized WhirlPak plastic bags (Nasco, Ft. Atkinson, WI). All fresh feces were immediately placed in an insulated foam container with dry ice, transported to the laboratory, and stored at 208C until further analysis.

AST Method: Broth Microdilution

Reference explicitly reports AST breakpoints: None

Reference reports using a MIC table: None