Reference | 2QC2FT5J (78)

Therefore, there is a need for cost-effective methods for evaluating resistance against colistin in mixed E. coli populations from animal fecal samples. Here, we designed and evaluated a broth microdilution-based method to quantify colistin resistance in E. coli populations from pooled chicken fecal samples. We then related the observed results to data on antimicrobial use (AMU) from the same flocks.

Fresh pooled fecal samples were collected from each flock at three time points: (i) day-old chicks, (ii) ;2- to 3-month-old chicks (midproduction), and (iii) ;4- to 6- month-old chicks (end of production). Day-old-chick fecal (i.e., meconium) samples were collected from the crates at the time when chicks were delivered to the farms. For midproduction and end-of-production sampling, sterile paper liners were placed near drinkers and feeders in the chicken house/pen to collect deposited droppings. After a minimum of 10 droppings had been deposited, liners were swabbed using sterile gauze. E

AST Method: Broth Microdilution

Reference explicitly reports AST breakpoints: None

Reference reports using a MIC table: None