Reference | N9IRTXW9 (46)

Detection and characterization of ESBL-producing Enterobacteriaceae from the gut of healthy chickens, Gallus gallus domesticus in rural Nepal: Dominance of CTX-M-15-non-ST131 Escherichia coli clones.


Hosuru Subramanya, Supram; Bairy, Indira; Nayak, Niranjan; Amberpet, Rajesh; Padukone, Shashiraj; Metok, Yang; Bhatta, Dharm Raj; Sathian, Brijesh (No Location)

None (2020)

Reference


The present study aimed to investigate the colonization proportion of EPE and CPE in the gut of healthy poultry, Gallus gallus domesticus in Kaski district, western Nepal. We also compared the rate and load of EPE isolates between healthy organically-raised backyard chickens and commercially grown chickens. Further, we investigated the distribution of ESBL genes, pandemic E. coli clonal group ST131 and co-resistance among the EPE isolates.

During the study period (October 2016 to January 2018), districts in Nepal were divided into political units called Village Development Committees (VDCs), and the metropolitan regions interquartile range. were divided into wards. Nepal was divided into 14 zones. The study area Kaski district is under the Gandaki zone. The sample size was determined based on prevalence rate, as reported by the previous study by Hussain et al. [15]. We applied the cluster random sampling method. All 47 VDCs of Kaski district was numbered based on alphabetic order, 23 (49%) VDCs were selected by random sample calculation using formula = RANDBETWEEN (1, 47). The random numbers generated were 1, 2, 4, 15, 40, 27, 16, 33, 31, 5, 22, 17, 37, 26, 45, 34, 23, 9, 41, 39, 8, 13, and 28. A total of 113 rectal swabs specimens from poultry birds were collected from 113 farms (66 backyard and 47 commercial farms) distributed over the Kaski district in western Nepal.

AST Method: Disk Diffusion

Reference explicitly reports AST breakpoints: None

Reference reports using a MIC table: None

Is Excluded: False

Country Sub-Region Sub-Region Detail
ID Note Resolution

Factors


Title Host Host Production Stage Description ROs
Production type Chicken Broilers Farm broiler vs. backyard 7