Reference | Tenhagen2020 (40001)

The study was carried out over the whole year in 2018 in the framework of a national monitoring program set up by the authorities of the Federal States, the Federal Office for Consumer Protection and Food Safety (BVL) and the German Federal Institute for Risk Assessment (BfR) in Berlin, Germany. The primary purpose of the monitoring was the determination of the exposure of consumers in Germany to zoonotic bacteria in the food chain, namely Campylobacter spp.

The number of samples to be collected was distributed across the country based on the human population in the respective federal states. The sample size was calculated to allow for an accuracy of –5% at an assumed prevalence of 50% leading to a target of 384 samples per population because the framework of the monitoring sample are examined for various bacterial species with diverse expected prevalences. However, as there were concerns regarding the availability of organic turkey meat at the retail outlets it was expected that for the organic meat the full sample size would not be achieved. Only one sample per production batch was to be collected per sampling point. However, because of decentralized sampling and primary isolation it cannot be excluded that samples from different retail points contained the same production badge. Samples were collected by the veterinary officials or their staff in retail markets all over Germany and transported under cooled conditions (2–8C) to the laboratory within 24 h. Samples were tested in the regional laboratories of the federal states according to ISO 10272-1:2017, procedure B and 25 g of meat were used as test portion (ISO, 2017).

AST Method: Broth Microdilution

Reference explicitly reports AST breakpoints: True

Reference reports using a MIC table: True