Reference | Price2005 (20015)

enrichment. Fresh chicken products from two antibiotic-free producers, Bell & Evans (Fredericksburg, PA; A) and Eberly Poultry (Stevens, PA; B), and two con? ventional producers, Perdue Farms (Salisbury, MD; C), and Tyson Foods (Springdale, AR; D), were purchased seven to eight times from grocery stores in the Baltimore, Maryland, area over the course of 10 weeks (from 25 February to 13 May 2003; Table 1). All samples were purchased in packaging at the processing plant, and all products were bone-in and skin-on (i.e., not skinless boneless products). On most occasions, three separate packages from the four producers were purchased each time; all three samples were purchased from the same grocer on a given date and were typically from the same produc? tion lot. Packages were refrigerated at 4?C until they were sampled (within 48 hr of purchase).

A single piece of chicken was sampled from each package. The package was wiped with 70% ethanol and cut open with a new disposable razor blade, and the plastic cover was removed and photocopied for record keeping. Sterile forceps were used to transfer a single piece of chicken to a stomacher bag containing 200 mL sterile Bolton broth sup? plemented with laked horse blood (Oxoid, Ogdensburg, NY; Quad Five, Ryegate, MT). Samples were then shaken by hand for 2 min. Chicken was removed using forceps, and the bag was sealed 1-2 cm above the top of the broth. Enrichments were incubated at 42?C for 22-26 hr.

AST Method: None

Reference explicitly reports AST breakpoints: True

Reference reports using a MIC table: False