Reference | McDermott2002 (20010)

Newly hatched broiler chicks from a commercial source were allocated randomly to 2 groups of 50 (sarafloxacin treated) or 25 (enrofloxacin treated) birds each. The chickens were reared under conventional conditions on pine-shave bedding with continuous lighting. Animals were fed a standard antibiotic-free broiler starter/grower diet, which was prepared on site, and were given access to feed and water ad libitum. To help insure that exogenous Campylobacter species were not introduced via the feed, 20 feed samples were collected at approximately equal intervals from the mixer exit stream immediately after preparation of feed and were cultured using standard methods [7]. Five fluoroquinolone-susceptible isolates were identified, A mixture containing an equal number of all 5 strains (
1 £ 106 cfu total) was given toeach bird as a 0.2-mL gavage deposited directly into the crop, using a long-tipped pipette. Since chickens are usually colonized with Campylobacter species after 2weeks of age, the birdswere inoculated orally at either 16 (sarafloxacin treated) or 24 (enrofloxacin treated) days of age. The birds remained free of Campylobacter species until deliberately infected. In each experiment, half the birds received fluoroquinolone-containing water beginning at 30 days of age, and the other half served as C. jejuni–colonized, nonmedicated controls.

In the sarafloxacin study, fecal samples were collected just before treatment (day 0) and 5, 7, 9, 12, 19, and 26 days after starting treatment. In the enrofloxacin study, fecal samples were collected just before treatment and 1, 3, 5, 12, and 21 days after starting treatment. In both studies, 50 freshly voided fecal samples (25 each from the treated and control groups) were collected on each sampling day. The 25 samples were combined into 5 composite samples of 5 individual samples each. This resulted in a total of 35 composite samples from the sarafloxacin-treated birds and 30 composite samples from the enrofloxacin-treated birds.

AST Method: None

Reference explicitly reports AST breakpoints: True

Reference reports using a MIC table: False