Reference | Logue2010 (20007)

A single flock of c. 30 000 (broad breasted white) turkeys was selected for use in this study. The flock was physically separated into two halves (containing c. 15 000 birds each) during the brooding phase (i.e. the first 4 weeks of development) in the brooder barn. Once the poults had reached 4 weeks of age, each half of the brooder barn was transferred into a separate finishing barn where the flock remained until slaughter. One half of the flock was A single flock of c. 30 000 (broad breasted white) turkeys was selected for use in this study. The flock was physically separated into two halves (containing c. 15 000 birds each) during the brooding phase (i.e. the first 4 weeks of development) in the brooder barn. Once the poults had reached 4 weeks of age, each half of the brooder barn was transferred into a separate finishing barn where the flock remained until slaughter. One half of the flock was designated as the untreated (control) group and received nonmedicated water, the other half was designated the treated group and received tylosin (Tylan Soluble; Elanco Animal Health, Greenfield, IN, USA) treatments during three separate dosing periods.

A total of 3252 samples were collected during this study as follows. At week 0, caecal swabs (n = 200) were taken to test for the presence of Campylobacter in the newly hatched poults. Faecal samples were collected on a weekly basis to recover Campylobacter isolates from turkeys: for weeks 1 through 5, 100 samples were collected in the control and treated barns; for weeks 6, 7 and 10, 50 samples were collected in the control barn and 100 in the treated barn; for week 11 and 12, 50 samples per barn were collected, for weeks 13 through 18, 50 samples were collected in the control barn and 100 in the treated barn. Samples were not collected during weeks 8 and 9. Environmental swabs of the brooder barn before placement (n = 20) and prior to placing in the finisher barns (n = 20 for control and n = 20 treated) were collected; samples were also collected from the finisher barns after the birds were removed for processing (n = 16 for each barn). Samples were collected at the processing facility where the turkeys were processed to final product. One hundred postchill carcass swabs were collected from carcasses using methods described previously (Logue et al. 2003a,b). Caecal contents were collected from birds at pre-evisceration (n = 100) by squeezing contents of the caecum into Whirl-Pack bags (Nasco, Fort Atkinson, WI). Samples were collected from each flock that was processed, i.e. control and treated birds. Finally, samples of the transport trucks were collected onsite pre (n = 5) and postwash, (n = 5) using Spongilces to swab an area of c. 100 cm2.

AST Method: None

Reference explicitly reports AST breakpoints: True

Reference reports using a MIC table: False