Reference | Jung2003 (20002)

Market age broilers 7 to 8 weeks of age were obtained from a local producer and housed in floor penwith pine shavings litter. Broilers were challenged with approximately 107 CFU of Salmonella Typhimurium per ml in trial 1 and with 109 CFU of Salmonella Typhimurium per ml in trial 2 via oral gavage and were divided into four groups. The first group (the control group) received no treatment, the second group received sodium nitrate (SN) treatment (574 mg of NaNO3 per kg of feed), the third group received ECP treatment (15 mM NaClO3 equivalents [i.e., this experimental product contained 15 mM chlorate ions]), and the fourth group received ECP treatment in combination with SN treatment. The ECP solution also contained 2.5mM sodium nitrate and 20 mM sodium lactate to induce nitrate reductase activity and to provide at least a minimal amount of available reductant. The SN treatment was administered via feed for the 5 days immediately prior to slaughter, and ECP was provided via ad libitum access to drinking water for the last 2 days before slaughter. Feed was removed 8 to 10 h before slaughter.

All broilers were killed by cervical dislocation. Cecal contents (0.5 g) were aseptically collected and deposited in 4.5 ml of phosphate buffer (pH 6.5). Samples were thoroughly mixed, serially diluted in phosphate buffer, and then spread plated onto brilliant green agar (BGA; Oxoid, Unipath Ltd., Basingstoke, Hampshire, UK) containing NN (BGA-NN).

AST Method: None

Reference explicitly reports AST breakpoints: None

Reference reports using a MIC table: None