Bibbal, D. and Dupouy, V. and Ferr, J. P. and Toutain, P. L. and Fayet, O. and Prre, M. F. and Bousquet-Mlou, A. (France)
Applied and Environmental Microbiology (2007)
Eighteen 7-week-old, commercial, healthy piglets that had never received antibiotics were used. They were housed separately in individual pens throughout all the experiments. A meal was given twice daily, and water was provided ad libitum. Ampicillin was administered once a day at 20 mg/kg of body weight for 7 days (from day 0 to day 6) following three modalities: the intramuscular route, the oral route in fasted pigs, or the oral route in fed pigs. The design schedule consisted of three successive series of six animals receiving ampicillin treatments as follows: intramuscular (n = 2), oral route under fed conditions (n = 2), and control without treatment (n = 2) in the first series; intramuscular (n = 2), oral route under fasted conditions (n = 2), and control without treatment (n = 2) in the second series; oral route under fed conditions (n = 2), oral route under fasted conditions (n = 2), and control without treatment (n = 2) in the third series. Six pigs were used in the control group and four pigs in each ampicillin treatment group. Intramuscular injections of ampicillin sodium (Ampicilline Cadril; Laboratory Coophavet, Ancenis, France) were administered in the neck. For oral routes, a medicinal premix (Ampicilline 80 Porc Franvet; Laboratory Franvet, Segre´, France) was dissolved in water and administered by gastric intubation. Fasted swine were starved 16 h before ampicillin administration and fed 4 h after ampicillin administration. Ampicillin was administered to fed pigs at the end of their morning meal.
For phenotypic evaluation of ampicillin resistance, 5g fecal samples were taken from each pig, by digital manipulation or immediately after spontaneous defecation, at days 0 (before ampicillin administration), 1, 4, and 7. For the quantification of blaTEM genes in feces by real-time PCR, feces of each pig were collected two or three times before the treatment. The value given for day 0 is the mean of these samplings. Feces were then collected each day from day 1 to day 7. Samples were obtained as already described.
AST Method: Broth Microdilution
Reference explicitly reports AST breakpoints: True
Reference reports using a MIC table: False
Is Excluded: False
| Country | Sub-Region | Sub-Region Detail |
|---|---|---|
| France | Haute-Garonne (Metropolitan department) | Toulouse |
| ID | Note | Resolution |
|---|
| Title | Host | Host | Production Stage | Description | ROs |
|---|---|---|---|---|---|
| Ampicillin Use (intramuscular) | Swine | Weaners | Farm | Ampicillin administered once a day for 7 days as ampicillin sodium (Ampicilline Cadril) in the neck (intramuscularly), at 20 mg/kg of body weight. Pigs were 7 weeks (49 days) old at start of trial. Control group did not receive ampicillin. Extracted data | 1 |
| Ampicillin Use (oral, fasted) | Swine | Weaners | Farm | Ampicillin administered once a day for 7 days via gastric intubation as a medicinal premix (Ampicilline 80 Porc Franvet) dissolved in water, at 20 mg/kg of body weight. Pigs were fasted 16 hours before ampicillin administration and fed 4 hours after ampic | 1 |
| Ampicillin Use (oral, fed) | Swine | Weaners | Farm | Ampicillin administered once a day for 7 days via gastric intubation as a medicinal premix (Ampicilline 80 Porc Franvet) dissolved in water, at 20 mg/kg of body weight, at the end of their morning meal. Control group did not receive ampicillin. Pigs were | 1 |