Awosile, Babafela and McClure, J. and Sanchez, Javier and Rodriguez-Lecompte, Juan Carlos and Keefe, Greg and Heider, Luke C. (Canada)
Journal of Dairy Science (2018)
This study was carried out to determine the frequen-cy of fecal carriage, antimicrobial susceptibility, and resistance genes in Salmonella enterica and Escherichia coli with reduced susceptibility to extended-spectrum cephalosporins (ESC) isolated from 488 dairy calves from 8 farms in New Brunswick, Canada. Both S. en-terica and E. coli with reduced susceptibility to ESC were isolated using selective culture. Minimum inhibi-tory concentrations to a panel of antimicrobial drugs were determined for randomly selected E. coli isolates and all of the Salmonella isolates. Multiplex PCR were conducted on the selected ESC-resistant E. coli to as-sess the β-lactamase resistance genes (blaCTX-M, blaCMY-2, blaSHV, and blaTEM) and plasmid-mediated qnrB and qnrS resistant genes. Information on ceftiofur use and other farm management practices were collected by the use of a questionnaire to determine the risk factors for the fecal recovery of E. coli with reduced susceptibil-ity to ESC.
A convenience sample of 8 dairy farms from New Brunswick was recruited. Farms were visited on a fortnightly basis by their regular herd veterinarians to collect rectal fecal swabs from Holstein dairy calves from July 2014 to February 2015.
AST Method: Broth Microdilution
Reference explicitly reports AST breakpoints: True
Reference reports using a MIC table: True
Is Excluded: False
| Country | Sub-Region | Sub-Region Detail |
|---|---|---|
| Canada | New Brunswick (Province) | None |
| ID | Note | Resolution |
|---|
| Title | Host | Host | Production Stage | Description | ROs |
|---|---|---|---|---|---|
| Antimicorbial use | Cattle | Pre-weaned Calves | Farm | Use of ceftiofur for anorexia/general infection | 1 |
| Pre weaned calve housing | Cattle | Pre-weaned Calves | Farm | Calf hutch vs. group | 1 |
| Antimicorbial use | Cattle | Pre-weaned Calves | Farm | Use of ceftiofur for scours | 1 |
| Antimicrobial use | Cattle | Pre-weaned Calves | Farm | Use of ceftiofur for lameness | 1 |
| Sick cows housed separately from other cows | Cattle | Pre-weaned Calves | Farm | Yes vs. no | 1 |
| Antimicorbial use | Cattle | Pre-weaned Calves | Farm | Florfenicol use | 2 |
| Lactating cow housing | Cattle | Pre-weaned Calves | Farm | Freestall vs. tiestall | 1 |
| Feeding of unpasteurized milk to calves | Cattle | Pre-weaned Calves | Farm | Yes vs. no | 1 |
| Frequency of ceftiofur use | Cattle | Pre-weaned Calves | Farm | Occasionally vs. regularly | 1 |
| Antimicrobial use | Cattle | Pre-weaned Calves | Farm | Use of ceftiofur for foot rot | 1 |
| Antimicorbial use | Cattle | Pre-weaned Calves | Farm | Tulathromycin use | 1 |
| No. of cows/herd | Cattle | Pre-weaned Calves | Farm | Small (<23) or Large (>/=23) | 1 |
| No. of calves/herd | Cattle | Pre-weaned Calves | Farm | Small (<23) or Large (>/=23) | 1 |
| Antimicorbial use | Cattle | Pre-weaned Calves | Farm | Trimethoprim-sulfadoxine use | 1 |
| Antimicrobial use | Cattle | Pre-weaned Calves | Farm | Regular ceftifur use on farm | 1 |
| Antimicrobial use | Cattle | Pre-weaned Calves | Farm | Use of ceftiofur for mastitis | 1 |
| Antimicorbial use | Cattle | Pre-weaned Calves | Farm | Use of ceftiofur for respiratory diseases | 1 |
| Antimicorbial use | Cattle | Pre-weaned Calves | Farm | Use of ceftiofur for metritis/retained placenta | 1 |