Aperce, C. C. and Amachawadi, R. and Van Bibber-Krueger, C. L. and Nagaraja, T. G. and Scott, H. M. and Vinasco-Torre, J. and Drouillard, J. S. (United States of America)
PLoS ONE (2016)
Twenty-six Holstein steers (568.8 ± 55 kg body weight) were housed in individual pens within three barns containing 5, 5, and 3 steers representing each treatment. Barns had concrete-surfaced pens (1.5 m × 6 m), were covered with corrugated roofing and equipped with individual feed bunks. Water fountains were shared between adjacent pens. Two treatments, a control and a menthol group, were randomly assigned to steers and were equally represented in each of the three barns. Crushed menthol (99.7% purity, Prinova USA LLC, Carol Stream, IL) was included at 0.3% on a dry matter basis in a basal diet consisting of 50% steam flaked corn, 33% corn gluten feed, and 10% corn silage. Diets were manufactured daily to avoid excess volatilization of menthol. Steers received 300 mg of monensin (Elanco Animal Health, Greenfield, IN) and 90 mg of tylosin (Elanco Animal Health) per animal daily and were fed ad libitum with free access to water for 30 days.
Fecal samples were obtained from each animal from the rectum, before feeding, on days 0 (before inclusion of menthol), 16, 23, and 30. Suspensions were made from 1 g of each sample.
AST Method: Broth Microdilution
Reference explicitly reports AST breakpoints: True
Reference reports using a MIC table: False
Is Excluded: False
| Country | Sub-Region | Sub-Region Detail |
|---|---|---|
| United States of America | Kansas (State) | None |
| ID | Note | Resolution |
|---|
| Title | Host | Host | Production Stage | Description | ROs |
|---|---|---|---|---|---|
| Menthol Diet Supplementation | Cattle | Finishers | Farm | Crushed menthol (0.3% dry matter) in a basal diet was fed ad libitum. Steers received 300 mg monensin, 90 mg tylosin per animal daily. Data extracted is from Day 30, and the total # of isolates represents the total # resistant to tetracycline (Table 1). | 2 |
| Menthol Diet Supplementation | Cattle | Finishers | Farm | Crushed menthol (99.7% purity) was included at 0.3% on a dry matter basis in a basal diet, fed ad libitum. Steers received 300 mg of monensin and 90 mg of tylosin per animal daily. Data extracted is from Day 30 (the final day). | 15 |