Reference | Zhang_2018_FrinMi (10338)

This study was conducted in a chicken farm with two different poultry houses (1000 m^2 each). The two houses were separated about 50 m to each other. After hatching, 50,000 chickens were equally and randomly allocated into two poultry houses (Day 0). Chickens in the medicated group (n = 25,000) were given successive prophylactic doses (0.5 mg/l) of apramycinsulfate in their drinking water from Days 1 to 5. In comparison, the un-medicated group (n = 25,000) was given drinking water without apramycin. No other antibiotics were used during the study period. Add antibiotic to drinking water for 5 days is the normal production behavior of the laying hens company.

Specifically, 15 cloacal swabs were collected from both the medicated and un-medicated groups at Day 0 and placed separately into sterile plastic bags. Fifteen sterilized plates were randomly placed under selected cages along two main diagonals of the poultry house containing both the medicated and un-medicated groups. Plates were placed at 12:00 am and withdrawn at 3:00 pm to allow for the collection of fresh fecal samples. Collections occurred on Days 1, 2, 3, 4, 5, 6, 8, 10, 15, 20, 30, and 40. Flies were captured using a sweep net on each sampling day from both of the two houses and approximately 30 flies were individually placed into sterile tubes for later morphological classification.

AST Method: Agar Dilution

Reference explicitly reports AST breakpoints: False

Reference reports using a MIC table: False