Reference | Tadesse_2011_FooPatandDis (10332)

Fecal and carcass swab samples were collectedfrom a total of 34 farm-slaughter pairs: 16 from region 1 (Ohioand Michigan) and 18 from region 2 (Wisconsin and Iowa).Ten of the farms from region 1 were from conventional pro-duction system and the remaining 6 were from ABF pigproduction system. Of the 18 farms included from region 2, 6were from conventional and 12 from ABF. The number of pigssampled per farm ranged between 10 and 34 for region 1, and19 and 32 for region 2. Sampling was conducted within a 3-year period between 2002 and 2005.

Ten grams of fecal sample was collected with a gloved handdirectly from the rectum of market age pigs within 48 hoursbefore slaughter. The same groups of pigs were followed tothe slaughter plant and carcass swabs were collected fromslaughter plants where the pigs were processed. One swabsample was collected from each carcass, and a total of 10carcasses per group were swabbed at pre- and posteviscera-tion stages. Sterile swabs soaked in 10 mL of buffered peptonewater (Becton Dickinson, Sparks, MD) were swiped along themidline of the carcass extending from the jowl to the ham. Atthe postchill stage, carcass swab samples were taken byswabbing a 100-cm2area at each of the three sample sites(ham, jowl, and belly) from 20 carcasses per group followingthe method recommended by U.S. Department of Agriculture(Palumboet al., 1999). This design resulted in a total of 40carcass swab samples from each group of pigs at the slaughterplants: 10 carcass swab samples each at pre-evisceration andpostevisceration, and 20 carcass swab samples at postchillstages. Samples were transported immediately to the labora-tory on ice and processed on the same day upon arrival.

AST Method: Agar Dilution

Reference explicitly reports AST breakpoints: False

Reference reports using a MIC table: False