Reference | Lebert_2018_ZooandPubHea (10308)

Smallholder flocks must not have a flock exceeding 300 birds and may hatch various poultry species. A list of provincially inspected abattoirs (n = 18) that process smallholder chickens, located within a 3-hr driving radius of Guelph, Ontario, were invited to participate. Five agreed to participate. Sampling was conducted between May and September in 2014 and 2015 during the main smallholder flock production season. Farm-level data was obtained by questionnaires that were distributed to producers by mail or administered in person at the abattoir if a producer was present and consented. The 2014 CIPARS abattoir data for Ontario on AMR for E. coli (n = 58) and Salmonella (n = 226) were used to compare AMR levels in smallholder flocks. Samples were obtained from slaughtered healthy chickens from seven federally registered abattoirs in Ontario.

During each sampling visit, five non-sequential viscera samples were collected from five chickens belonging to one flock and placed into individual leak-proof zipper bags. Caeca were separated out from the viscera upon arrival to the laboratory and caecal contents aseptically extracted.

AST Method: Broth Microdilution

Reference explicitly reports AST breakpoints: True

Reference reports using a MIC table: False