Reference | Jacobs.Reitsma_1994_LeinApMi (10295)

The induction of quinolone resistance in Campylobacter bacteria in broilers by quinolone treatment.


Jacobs-Reitsma, W.F. and Kan, C.A. and Bolder, N.M. (Netherlands)

Letters in Applied Microbiology (1994)

Reference


Day-old broiler chicks (Cobb) were obtained from a commercial hatchery. Eight groups of 15 chicks each were housed in floor-pens on litter. All groups were kept in separate rooms. Quinolone treatment of the chickens lasted 4 d. Treatment of group 6 started at day 1 and treatment of groups 2-5 and 7 started at day 26. Campylobacter challenge took place on day 19. All birds in groups 1-6 were individually challenged with 0.1 ml of saline solution containing 6 x 10’ cfu ml-’ Campylobacter jejuni. All birds in groups 7 and 8 were individually challenged with 0.1 ml of sterile saline solution (Campylobacter-free controls)

All groups were tested for the presence of Campylobacter on day 12, 19, 26 and 33 by individual cloacal swabbing. All chickens were slaughtered on day 43, and from each bird caecal material was aseptically sampled with a sterile swab.

AST Method: Disk Diffusion

Reference explicitly reports AST breakpoints: True

Reference reports using a MIC table: False

Is Excluded: False

Country Sub-Region Sub-Region Detail
Netherlands None None
ID Note Resolution

Factors


Title Host Host Production Stage Description ROs
Enrofloxacin Use Chicken Chicks Farm Tx group challenged with Camp. Jejuni on day 19. Tx with 15ppm enrofloxacin on days 26-29. Sampling on day 43. Control group also challenged on day 19 but received no tx. 4
Enrofloxacin Use Chicken Chicks Farm Tx group challenged with Camp. Jejuni on day 19. Tx with 50ppm enrofloxacin on days 1-4. Sampling on day 43. Control group also challenged on day 19 but received no tx. 4
Enrofloxacin Use Chicken Chicks Farm Tx group challenged with Camp. Jejuni on day 19. Tx with 50ppm enrofloxacin on days 26-29. Sampling on day 43. Control group also challenged on day 19 but received no tx. 4
Flumequine Use Chicken Chicks Farm Tx group challenged with Camp. Jejuni on day 19. Tx with 15ppm flumequine on days 26-29. Sampling on day 43. Control group also challenged on day 19 but received no tx. 4
Flumequine Use Chicken Chicks Farm Tx group challenged with Camp. Jejuni on day 19. Tx with 50ppm flumequine on days 26-29. Sampling on day 43. Control group also challenged on day 19 but received no tx. 4