Reference | Hoogenboom_2008_FAaC.PACACEaRA (10292)

In the case of pigs, 31 organic pig farms were visited, ten in December 2003–January 2004 and 21 in April– May 2005. Samples of bovine faeces were collected at ten organic farms according to routine procedures of the Food and Consumer Products Safety Authority. The ten farms were visited in October–November 2003. One farm with a positive result on E. coli O157 was revisited in June 2004. To examine Salmonella in faeces of laying hens and broilers, respectively, ten (April–May 2004) and nine (April–June 2005) organic farms were visited.

For analysis on Salmonella and E. coli O157 in pigs, at each farm faeces sampled from twelve pigs, shortly before slaughter, were pooled into a combined sample. On farms with more stables, two or three pooled samples were taken. To study the incidence of antibiotic-resistant bacteria, faecal samples were taken from five pigs per farm. Pig kidneys and diaphragm from 20 animals of 20 different organic farms were sampled at the slaughterhouse. For cattle, each pooled sample consisted of faeces from twelve cows. The number of samples per farm was two for fewer than 40 cows, three for 40–59 cows, four for 60–199 cows and five for more than 200 cows. Ten bovine kidneys from ten organic farms were sampled at the slaughterhouse. For laying hens and broilers, five pooled samples, each from twelve fresh droppings, were collected from each farm. For antibiotic-resistant bacteria in broilers, five samples of one fresh dropping per farm were collected and send to the laboratory, according to standard protocols.

AST Method: Agar Dilution

Reference explicitly reports AST breakpoints: False

Reference reports using a MIC table: False