Reference | Hinton_1986_JoofAnCh (10291)

One-day-old chicks were purchased from a commercial hatchery and groups of ten birds were kept in solid-sided fibre glass tubs (Kaukas, Hinton & Linton, 1986). Food and water were available ad libitum. A commercial chick starter ration, which contained the growth promoting antibiotic nitrovin (10 mg/kg) and the coccidiostat monensin (100 mg/kg) was fed for the first 14 days. A broiler ration containing virginiamycin (20 mg/kg) and monensin was used for the remainder of the experimental period which terminated when the birds were 23 days of age. Each of the two experiments was performed on two separate occasions and each involved one group of chickens that was dosed with an antibiotic in the water for five days and one which acted as an 'undosed' control. In experiment 1, one group of birds was dosed with oxytetracycline in the water (180 mg/l) for five days, commencing after the fourth swab had been taken. Fresh antibiotic solution was offered each day. In experiment 2 the birds were similarly dosed with tylosin (550 mg/l).

Swabs of cloacal faeces were obtained from five of the birds in each group on the day of arrival and then from the same five birds every other day until day 23.

AST Method: Selective Media

Reference explicitly reports AST breakpoints: Uncertain

Reference reports using a MIC table: False