Reference | Cassenego_2011_BrJoofMi (10262)

Species distribution and antimicrobial susceptibility of enterococci isolated from broilers infected experimentally with Eimeria spp and fed with diets containing different supplements.


Cassenego, A.P.V. and D'Azevedo, P.A. and Ribeiro, A.M.L. and Frazzon, J. and Sand, S.T.Van Der and Frazzon, A. P. G. (Brazil)

Brazilian Journal of Microbiology (2011)

Reference


The study was conducted using a total of 250 day-old male Cobb 500. The broilers were housed indoors and distributed in a completely randomized design divided into eight treatment groups with five replicates each (12 birds/box). Broilers were fed with a diet based on corn-soybean meal, vegetable oil, minerals and vitamins and supplemented with a combination of ionophore-coccidiostatics (monensin) at 90g/ton, probiotic (Enterococus faecium 3.5 x 10^10 UFC/g) at 35g/ton, essential oil (extracted of thyme and clove) at 100g/ton and/or antimicrobial (bacitracin methylene disalicylate 11%) at 200g/ton. At 14 days old, all birds, except the control, received a solution containing 5x10^4 and 1x10^4 oocysts/chicken of Eimeria maxima and Eimeria acervulina, respectively. The broilers were maintained until 28 days old. The study groups can be summarized as follows: 1) unmedicated infected control; 2) ionophore coccidiostatic infected group; 3): probiotic infected group; 4) ionophore coccidiostatic and probiotic infected group; 5) essential oil infected group; 6) ionophore coccidiostatic and essential oil infected group; 7) growth promoter infected group; 8) unmedicated uninfected control.

Five cloacal swabs samples from each group were collected and environmental contamination was avoided.

AST Method: Disk Diffusion

Reference explicitly reports AST breakpoints: False

Reference reports using a MIC table: False

Is Excluded: False

Country Sub-Region Sub-Region Detail
Brazil Rio Grande do Sul (State) Porto Alegre, Rio Grande do Sul
ID Note Resolution

Factors


Title Host Host Production Stage Description ROs
Diet Supplementation Chicken Broilers Farm Group 2 (diet supplemented with ionophore-coccidiostatics (monensin) at 90g/ton) vs Group 1 (un-supplemented diet). Both groups were inoculated at 14 days old with 5x10^4 and 1x10^4 oocysts/chicken of Eimeria maxima and Eimeria acervulina, respectively. 8
Diet Supplementation Chicken Broilers Farm Group 3 (diet supplemented with probiotic E. faecium 3.5x10^10 UFC/g at 35g/ton) vs Group 1 (un-supplemented diet). Both groups were inoculated at 14 days old with 5x10^4 and 1x10^4 oocysts/chicken of Eimeria maxima and Eimeria acervulina, respectively. 10
Diet Supplementation Chicken Broilers Farm Group 4 (diet supplemented with both monensin and probiotic--see previous factors for detailed dose info) vs Group 1 (un-supplemented). Both groups inoculated at 14 days old with 5x10^4 and 1x10^4 oocysts/chicken of Eimeria maxima and Eimeria acervulina. 10
Diet Supplementation Chicken None Farm Group 2 (diet supplemented with ionophore-coccidiostatics (monensin) at 90g/ton) vs Group 1 (un-supplemented diet). Both groups were inoculated at 14 days old with 5x10^4 and 1x10^4 oocysts/chicken of Eimeria maxima and Eimeria acervulina, respectively. 2
Bacitracin Use Chicken Broilers Farm Group 7 (in-feed bacitracin methylene disalicylate 11% at 200g/ton) vs Group 1 (regular feed). Both groups were inoculated at 14 days old with 5x10^4 and 1x10^4 oocysts/chicken of Eimeria maxima and Eimeria acervulina. 10
Diet Supplementation Chicken Broilers Farm Group 5 (diet supplemented with essential oil (extracted of thyme and clove) at 100g/ton) vs Group 1 (un-supplemented). Both groups were inoculated at 14 days old with 5x10^4 and 1x10^4 oocysts/chicken of Eimeria maxima and Eimeria acervulina. 10
Diet Supplementation Chicken Broilers Farm Group 6 (diet supplemented with both monensin and essential oil--see previous factors for dose info) vs Group 1 (un-supplemented). Both groups were inoculated at 14 days old with 5x10^4 and 1x10^4 oocysts/chicken of Eimeria maxima and Eimeria acervulina. 10