Reference | Brower_2017_EnviHealPers (10254)

The prevalence of extended-spectrum beta-lactamase-producing multidrug-resistant Escherichia coli in poultry chickens and variation according to farming practices in Punjab, India.


Brower, Charles H. and Mandal, Siddhartha and Hayer, Shivdeep and Sran, Mandeep and Zehra, Asima and Patel, Sunny J. and Kaur, Ravneet and Chatterjee, Leena and Mishra, Savita and Das, B. R. and Singh, Parminder and Singh, Randhir and Gill, J. P.S. and La (India)

Environmental Health Perspectives (2017)

Reference


Eighteen poultry farms (nine layers and nine broilers) were randomly selected from a list of farms in Punjab. In this cross-sectional design, all samples were collected over a 5-month period from July to November 2014. 1,556 Escherichia coli isolates from 530 birds were tested for susceptibility to 11 antimicrobials using the disk diffusion method and validated using VITEK2. After sampling was complete, a 30-min structured interview covering sanitation, disease control, and antimicrobial use practices was conducted with each farm manager.

Sixty cloacal swabs were collected from 30 birds selected at random (two swabs per bird) from each farm. 530 birds (n=530) were sampled for general susceptibility testing of E. coli, and 510 birds (n=510) were sampled for ESBL-producing Enterobacteriaceae. Since three E. coli isolates were sampled per bird, a total of 1,556 viable E. coli isolates (n=1,556) were tested for susceptibility against 11 antimicrobials.

AST Method: Disk Diffusion

Reference explicitly reports AST breakpoints: False

Reference reports using a MIC table: False

Is Excluded: False

Country Sub-Region Sub-Region Detail
India Punjab (State) None
ID Note Resolution

Factors


Title Host Host Production Stage Description ROs
Use of antimicrobials as growth promoters Chicken Broilers Farm Self-reported through survey 8
Use of antimicrobials as growth promoters Chicken Broilers Farm Self-reported through survey 1
Use of antimicrobials as growth promoters Chicken Layers Farm Self-reported through survey 10