Reference | Amachawadi_2011_AppandEnvMic (10241)

Selection of fecal enterococci exhibiting tcrB-mediated copper resistance in pigs fed diets supplemented with copper.


Amachawadi, R. G. and Shelton, N. W. and Shi, X. and Vinasco, J. and Dritz, S. S. and Tokach, M. D. and Nelssen, J. L. and Scott, H. M. and Nagaraja, T. G. (United States of America)

Applied and Environmental Microbiology (2011)

Reference


Sixty newly weaned piglets (21 days old) with an average body weight of 7.0 kg ( 1 kg) were randomly allocated to two dietary treatments. The two dietary treatments were as follows: basal diet with 16.5 ppm of copper (control group) or basal diet supplemented with 125 ppm of copper as copper sulfate (copper group). The basal diet consisted of corn, soybean meal, vitamins, amino acids, and trace mineral supplements, and piglets were housed in an environmentally controlled nursery facility. Each treatment group had a total of 30 piglets assigned to 5 pens with 6 piglets per pen. Piglets were fed treatment diets for 42 days.

Fecal samples were collected randomly from 3 pigs in each pen on days 0, 14, 28, and 42, and placed in individual bags. 1 g of feces from each sample was used for further analyses.

AST Method: Multiple Methods

Reference explicitly reports AST breakpoints: Uncertain

Reference reports using a MIC table: False

Is Excluded: False

Country Sub-Region Sub-Region Detail
United States of America Kansas (State) Manhattan
ID Note Resolution

Factors


Title Host Host Production Stage Description ROs
Diet Supplementation Swine Weaners Farm Control group: 16.5 ppm in-feed copper, Treatment group: 125 ppm in-feed copper sulfate. AMR data is reported as the # of isolates positive for the tcrB gene (determined from PCR). Data are representative of sampling day 42. 1