Amachawadi, R. G. and Scott, H. M. and Alvarado, C. A. and Mainini, T. R. and Vinasco, J. and Drouillard, J. S. and Nagaraja, T. G. (United States of America)
Applied and Environmental Microbiology (2013)
Crossbred yearling heifers (n=261) were blocked by initial body weight into heavy (330 kg)- and light (280 kg)-weight groups and assigned randomly to 4 pens with 10 or 11 heifers per pen. The pens were assigned, in a randomized complete block design, to a 2 by 2 factorial arrangement of treatmentsof dietary copper, normal (10 mg/kg of feed) or elevated (100 mg/kg of feed) and a commercial nutritional supplement (Linpro; Oleet Processing, Ltd., Regina, Saskatchewan, Canada) at 0 or 10% of the diet on a dry matter (DM) basis.
Fresh pen-floor fecal samples were collected from each pen (day 116 from heavy weight heifers before shipping for slaughter and day 131 from light weight heifers) and cultured for Enterococcus. PCR was used for resistant gene detection.
AST Method: None
Reference explicitly reports AST breakpoints: True
Reference reports using a MIC table: True
Is Excluded: False
| Country | Sub-Region | Sub-Region Detail |
|---|---|---|
| United States of America | Kansas (State) | None |
| ID | Note | Resolution |
|---|
| Title | Host | Host | Production Stage | Description | ROs |
|---|---|---|---|---|---|
| Copper supplementation | Cattle | None | Feedlot | Enterococcal isolates from cattle fed 100 mg/kg dietary copper in the diet (10X normal). Data are prevalence for copper resistance gene tcrB. Day 116 Only | 1 |
| Copper supplementation | Cattle | Beef Heifers | Feedlot | Enterococcal isolates from cattle fed 100 mg/kg dietary copper in the diet (10X normal). Data are prevalence for erythromycin resistance gene erm(B). Day 116 Only | 1 |
| Copper supplementation | Cattle | Beef Heifers | Feedlot | Enterococcal isolates from cattle fed 100 mg/kg dietary copper in the diet (10X normal). Data are prevalence for tetracycline resistance gene tet(M). Day 116 Only | 1 |