Reference | Aarestrup_2000_MicrDrugResi_1 (10217)

As part of the continuous surveillance for antimicrobial resistance in Denmark, E. faecium isolates are routinely isolated from fecal samples from healthy broilers and pigs and tested for their susceptibility to antimicrobial agents as previously described(5). In 1997, a total of 211 E. faecium isolates were recovered from broilers and 55 from pigs. These isolates, each representing different herds or flocks, were included in the present study. A total of 59 isolates of E. faecium from Norwegian animal husbandry were included in the study. Fifty-five isolates originated from fecal samples from 47 poultry farms (turkey and broiler farms) collected between June 1995 and January 1997. Eight poultry farms were represented with two isolates, because samples were obtained twice from these farms; each sample represented a different flock. Four isolates originated from fecal samples obtained from Norwegian pig farms between August 1995 and November 1996; each isolate represented one farm. From Finland, a total of 52 isolates were obtained from poultry fecal samples and a total of 43 isolates from swine fecal samples. Each isolate represented one farm. The fecal samples were collected between June and August 1996 as part of Finnish surveillance program.

Individual fecal samples. In Denmark, all samples were inoculated on Slanetz and Bartley agar and incubated for 2 days at 37°C, and typical colonies were identical to species level using phenotypic characteristics. The Norwegian isolates were obtained by cultivation on Slanetz and Bartley agar containing 50 ug/ml vancomycin. From Finland, samples were cultivated using Slanetz and Bartley agar.

AST Method: Agar Dilution

Reference explicitly reports AST breakpoints: True

Reference reports using a MIC table: True