Reference | Fleury2015 (10185)

Six animal rooms were used to house 48 Large White piglets obtained from the SPF herd at the ANSES Ploufragan laboratory. The piglets of 7 weeks of age were the progeny of five different sows and were randomized before the experiment. Each room contained eight piglets placed in two pens of four animals. The experimental groups were as follows: the nontreated (NT) control group (8 pigs) in room 1A, the ceftiofur-treated (T) group (8 pigs) in room 1B, the E. coli M63-inoculated group (M63) in rooms 1C (8 pigs) and 2C (8 pigs), and the E. coli M63-inoculated ceftiofur-treated group (M63-T) in rooms 1D (8 pigs) and 2D (8 pigs). The animals did not receive any treatment with any antibiotics prior to the assay. The same nonsupplemented starter diet was offered to the animals up to D2, and then pigs were fed with a nonsupplemented standard growing diet until the end of the experiment. E. coli M63 inoculations were performed on D0. Each piglet from groups M63 and M63-T was orally given a 10-ml suspension prepared from E. coli M63 cultivated on MH agar containing cefotaxime (2 mg/liter). On D0, just after E. coli M63 inoculation, piglets from groups T and M63-T were given a single intramuscular injection of a vegetable oil-based suspension of ceftiofur crystalline free acid (Naxcel; Pfizer) at the recommended dose of 5 mg/kg of body weight.

Individual fecal samples were collected from all animals from the day before inoculation (D−1) and on D1, D3, D7, D10, D13, D17, D22, and D28.

AST Method: None

Reference explicitly reports AST breakpoints: True

Reference reports using a MIC table: True