Reference | Ciesinski2018 (10144) (Excluded)

High dietary zinc feeding promotes persistence of multi-resistant E. coli in the swine gut.


Ciesinski, Lisa; Guenther, Sebastian; Pieper, Robert; Kalisch, Martin; Bednorz, Carmen; Wieler, Lothar H. (Germany)

PLoS ONE (2018)

Reference


32 landrace piglets were weaned 25±1 days after birth and randomly allocated to two different feeding groups balancing for gender, litter, and body weight. Corn starch was partially replaced by analytical grade zinc oxide (Sigma Aldrich, Taufkirchen, Germany), which was determined by atomic absorption spectrometry (AAS vario 6 spectrometer; Analytik Jena, Jena, Germany) resulting in a final concentration of 72 mg zinc/kg diet (control) and 2103 mg/kg (zinc group) respectively. No antimicrobial substances were administered to the piglets or their mothers.

At the age of 38 days (two weeks after weaning) feces were collected of all 32 piglets. For 16 of the 32 animals (8 per feeding group) an additional digesta and mucosa sample per piglet were collected after the animals were sacrificed. At the age of 52 days (four weeks after weaning) feces of the remaining 16 animals were collected and after the animals were sacrificed, again one digesta and one mucosa sample per animal were collected.

AST Method: None

Reference explicitly reports AST breakpoints: False

Reference reports using a MIC table: False

Is Excluded: True

Country Sub-Region Sub-Region Detail
Germany Berlin (Land) None
ID Note Resolution

Factors


Title Host Host Production Stage Description ROs
Zinc use Swine Weaners Farm Corn starch in feed was partially replaced by analytical grade zinc oxide: 72 mg zinc/kg diet (control) & 2103 mg/kg (zinc group). Multi-resistant isolates (resistant to at least 3 antimicrobials) taken from feces of pigs at 52 days of age. 4