Reference | Yun2017 (10136)

Piglets were weaned at approximately 28 +/- 3 days. Pigs were transported to a temperature-controlled room at weaning. Each room had 20 pens with approximately 20 to 25 pigs per pen (2.5 - 5.0 m). A total of 480 litters, producing 7156 piglets, were assigned to two groups during the first day after birth - ANT (amoxicillin treatment - 6 batches) or CON (non-treatment - 6 batches). A total of 3661 pigs were given an individually numbered green ear tag on their left ear and injected with 75 mg of amoxicillin in their neck muscle. A total of 3495 pigs were given an individually numbered orange ear tag on their right ear and not treated with any routine antibiotics at birth. At weaning, the piglets in the same treatment groups were housed in the same pens. The farm personnel treated piglet diseases according to the advice of the health care veterinarian of the herd. The E. coli epidemiological cut-off values (ECOFFs), determined by the European Committee on Antimicrobial Susceptibility Testing were applied to differentiate between susceptible and resistant populations.

Approximately three days after weaning, in a total of ten batches, approximately 5 g of fresh faeces were collected from three to four areas of the floors of each pen.

AST Method: Broth Microdilution

Reference explicitly reports AST breakpoints: False

Reference reports using a MIC table: False