Reference | Wiuff2003 (10134)

Forty healthy pigs at the age of 8-10 weeks weighing20-27 kg obtained from a single farm were allocatedinto 10 groups of four pigs each. Rifampicin resistantS. typhimuriumno. (pig isolate130302gla with O-antigens 1, 2, 5 and 12) and a nalidixicacid resistant mutant selected from this strain were usedfor inoculation of the pigs. The rifampicin resistantstrains were used to enable discrimination between theinoculated strain and other unwantedSalmonellastrains. Baytril 50 mg/ml enrofloxacin injectable solution(provided by Bayer Denmark A/S) was used for i.m.administration. For p.o. administration the 50 mg/mlsolution was diluted in Milli Q water immediately beforeuse to give a final concentration of 15 mg/ml. The pigs were inoculated orally with the mixed cul-ture of the twoSalmonellastrains by gastric intubationwith 5 ml of the inoculation solution. Each pig receivedapproximately 10^10 bacteria of the original quinoloneresistant strain whereof 10^8 bacteria were nalidixic acidresistant. Pigs were treated with 2.5 mg/kg bwt enrofloxacin by eitherintramuscular (i.m.) or oral (p.o.) administration at time points either 4 or 24 h after the infection.

Rectal faeces samples were takenafter the end of treatment every day for 11 days. Heparinized bloodsamples were taken from vena cava of each treated pig 2h after the first administration and 2 h after the thirdadministration (on the third day).

AST Method: Agar Dilution

Reference explicitly reports AST breakpoints: False

Reference reports using a MIC table: False