Reference | Sapkota2011 (10102)

The objective of this study was to prospectively evaluate the prevalence of antibiotic-resistant enterococci on large-scale conventional poultry farms that transitioned to organic practices. Two types of poultry farms were included: large-scale conventional broiler farms that were maintaining conventional practices and using antibiotics (n = 5), and large-scale (previously conventional) broiler farms that had just received organic certification and were producing their first flock of certified organic broilers (n = 5). Two individual poultry houses from each farm were included in the study, for a total of 20 poultry houses. The specific antimicrobials that were used in feed in the conventional poultry houses were as follows: bacitracin (50 g/ton), virginiamycin (15 g/ton), roxarsone (45.35 g/ton), salinomycin (60 g/ton), nicarbazin (0.0125%), and decoquinate (27.2 g/ton).

From March to June 2008, poultry litter, water, and feed samples were aseptically collected from the conventional and newly organic poultry houses. Litter samples (500 g) from the top 11-5 cm of litter were collected from three randomly selected areas of each poultry house.

AST Method: Broth Microdilution

Reference explicitly reports AST breakpoints: True

Reference reports using a MIC table: True