Reference | Ozaki2011 (10080)

Sampling was carried out in 4 broiler farms located ineastern Japan. Birds in 2 of the 4 farms were not given anyantimicrobials throughout the rearing period. In these 2farms (farm 1 and farm 2), antimicrobials had not beenused in the rearing practice for more than 1 year.Antimicrobials were administered birds in the remaining2 farms (farms 3 and 4) for treating diseases associatedwith bacteria, mycoplasma, and coccidia. In farm 3,200 ppm of oxytetracycline (OTC), 500 ppm of sulfadi-methoxine (SDMX), and 300 ppm of tylosin were addedinto feed at 14 days of age and successively given for 7days. On farm 4, 500 ppm of SDMX was added into feed at20 days of age and given successively for 3 days. OTC andSDMX had been used over the past year in all flocks rearedon farms 3 and 4, respectively. Flocks in farms 1, 2, and 4were raised in open-type conventional chicken houseswhile the flock in farm 3 was raised in an environmentallycontrolled house

Fecal samples were collected from the birds atthe ages of 2–3 days, 14–17 days (in the case offarms 3 and 4, before the antimicrobials were given),and 47–50 days. Ten samples of freshly dropped feceswere obtained at points distant from one another ineach of the chicken houses.

AST Method: Agar Dilution

Reference explicitly reports AST breakpoints: True

Reference reports using a MIC table: True