Reference | Lugsomya2018a (10044)

Antimicrobial resistance in commensal Escherichia coli isolated from pigs and pork derived from farms either routinely using or not using in-feed antimicrobials.


Lugsomya, Kittitat; Yindee, Jitrapa; Niyomtham, Waree; Tribuddharat, Chanwit; Tummaruk, Padet; Hampson, David J.; Prapasarakul, Nuvee (Thailand)

Microbial Drug Resistance (2018)

Reference


2 farms: farm A (using antibiotics) and farm NA (not using antibiotics). For each farm, one healthy pig from each of ten different litters was selected for use in the experiment (a total 10 pigs per group). In farm A, a combination of tiamulin and amoxicillin at 100 ppm and 250 ppm, respectively, were routinely mixed into the feed during the postweaning nursery and growing periods. In farm NA, antimicrobials had not been used as a feed additive for over 10 years.

Each pig sampled at five periods up to and including postslaughter: the neonatal period (at 5 days of age); the postweaning nursery period (at 8 weeks of age); the growing period (at 14 weeks of age); the finishing period (at 24 weeks of age, just before transport to the abattoir); and following slaughter at the abattoir. For the pigs in the neonatal and nursery periods, rectal swabs were taken directly. For pigs in the growing and finishing periods, at least 25 g of rectal feces were collected. For the pigs at the abattoir, meat (“pork”) was sampled.

AST Method: Disk Diffusion

Reference explicitly reports AST breakpoints: False

Reference reports using a MIC table: None

Is Excluded: False

Country Sub-Region Sub-Region Detail
Thailand Other (Other) Chainart Province
Thailand Other (Other) Nakhon Pathom Province
ID Note Resolution

Factors


Title Host Host Production Stage Description ROs
Combination of tiamulin and amoxicillin use Swine Weaners Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed. Resistance outcome = blaCTX-M-25/26 group gene. 1
Combination of tiamulin and amoxicillin use Swine Grower-finisher Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed. Resistance outcome = blaCTX-M-25/26 group gene. 1
Combination of tiamulin and amoxicillin use Swine Grower-finisher Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed. 31
Combination of tiamulin and amoxicillin use Swine Grower-finisher Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed. Resistance outcome = ESBL. 1
Combination of tiamulin and amoxicillin use Swine Weaners Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed. 34
Combination of tiamulin and amoxicillin use Swine Grower-finisher Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed. Resistance outcome = blaPSE-1 gene. 1
Combination of tiamulin and amoxicillin use Swine Grower-finisher Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed. Resistance outcome = imipenem. 1
Combination of tiamulin and amoxicillin use Swine Weaners Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed. Resistance outcome = ESBL. 1
Combination of tiamulin and amoxicillin use Swine Weaners Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed. Resistance outcome = blaPSE-1 gene. 1
Combination of tiamulin and amoxicillin use Swine Weaners Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed. Resistance outcome = imipenem. 1
Combination of tiamulin and amoxicillin use Swine Grower-finisher Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed (sample at grower period) 5
Combination of tiamulin and amoxicillin use Swine Weaners Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed 1
Combination of tiamulin and amoxicillin use Swine Weaners Farm Tiamulin at 100 ppm, amoxicillin at 250 ppm in feed. Resistance outcome = blaCTX-M-2 group gene. 1