Reference | Khachatryan2004 (10021)

To assess the impact of the TM-50-supplemented diet on the prevalence of resistant bacteria, we performed the following experiment. Newborn calves were consecutively assigned to either the control or experimental group. Calves were physically separated from each other in individual pens, and control and treatment group calves were held in different locations within the calf facility to avoid any chance of physical contact. Calves in the control group (n = 9) were fed bulk milk twice daily, which was supplemented once daily but contained no TM-50 (−TM-50). The diet of the experimental group (n = 9) was identical except it included TM-50 in the milk supplement (+TM-50). The calves left the experiment at 12 weeks of age. The calf diet included, in addition to the milk, a grain concentrate free of antimicrobials. Calves were weaned at 4 to 6 weeks of age. There were no documented instances of use of other antimicrobial drugs (therapeutic) during this study.

Fresh fecal samples (20 g) were taken two to three times per week from each calf.

AST Method: Multiple Methods

Reference explicitly reports AST breakpoints: True

Reference reports using a MIC table: False